15 Days & 30 Days E Training – Training on Molecular Diagnostics: From Library to Laboratory E Skilling
Online Programme
Has the process of Molecular Diagnostics ever intrigued you? It is the hottest topic right now among this pandemic from other orthodox topics. What if we told you that YOU can learn how to operate and analyze the latest techniques of molecular diagnostics? Limited seats remain.
Course Content:-
PHASE 1 (Day 1 to Day 8)
Day 1:
·
Plasmid DNA Isolation
·
Competent Cell Preparation
·
Transformation
·
Genomic DNA Isolation
Day 2:
·
RNA isolation
·
Restriction Digestion & Ligation
·
PCR and its importance with Demo
·
Primer Design, Fasta, Blast
Day 3:
·
RT PCR
·
Analysis after RT PCR
·
Qualitative methods: densitometric analysis
Day 4:
·
What are Cloning and its importance
·
What are the factors needed for cloning?
·
The idea about restriction sites and restriction enzyme
·
Idea about vectors
·
Uses of PCR during cloning
·
In silico cloning techniques
Day 5:
·
What is next after cloning?
·
Colony PCR
·
Plasmid DNA isolation
·
Restriction checking
·
Sequencing
·
Different sequencing methods
Day 6:
·
SDM
·
Methods for SDM
·
Methods and requirements for protein purificationDay 7:
· Remaining methods for protein purification
· Protein estimation techniques
· About a special protein purification process
· Hybridization technique
Day 8:
· CRISPR Cas9 system
· How you can use it in your work
· SgRNA preparation
· Chromatographic technique
PHASE 2:-
Day 9-14:
History of DNA sequencing Technologies: Maxam-Gilbert Method, Automated DNA Sequencing, Human Genome Project, Technologies involved in sequencing: PCR based sequencing/Non-PCR based Sequencing
Clinical Molecular Diagnostic technique: Targeted Analyses (Restriction Fragment Length polymorphism, Allele-Specific Oligonucleotide Hybridization, Amplification Refractory Mutation System etc), Detection of Unknown Mutation Studies (Gradient Gel Electrophoresis, Single-Strand Conformation Polymorphism and Heteroduplex Analyses, Denaturing High-Performance Liquid Chromatography), Detection of Copy Number Variations(Southern Blotting, Multiplex Ligation-Dependent Probe Amplifi cation (MLPA), Array Comparative Genomic Hybridization (aCGH) etc), Pitfalls of Conventional PCR-Based Methods.
The Technologies and Bioinformatics: Methods of Gene Enrichment and Massively Parallel Sequencing Technologies; Target Gene Enrichment Methods, PCR based Methods and Capture-Based Enrichment: By Hybridization with Oligonucleotide Probes, Overview of Massively Parallel Sequencing Technologies: Second-Generation Sequencing Technologies and A Glance at the Third-Generation Sequencing Technologies
Sequence Alignment, Analysis, and Bioinformatic Pipelines: Historics (NGS Platforms, Paradigm Shift Enabled by NGS), Informatics in NGS Analysis (Pipeline Overview, Alignment
Starting:- 15th August 2020
Registration Link:- https://docs.google.com/forms/d/e/1FAIpQLSeStUnEHPvyI8JzCSLF4jRbUL3tikVhxZbSCMHho88qh0TYig/viewform
Payment:
15 DAYS INR 4000
30 DAYS INR 6000
Payment Bank Details
SHRM Biotechnologies Pvt Ltd
Account No:- 919020085724614
IFSC Code :- UTIB0001707
Axis Bank (Salt Lake Sector II)
If you need link for Debit / Credit Card, kindly inform us