Course Overview: This course provides an in-depth understanding of molecular biology principles, techniques, and applications in research, medicine, and biotechnology. Participants will explore the structure and function of nucleic acids (DNA and RNA), proteins, and the molecular mechanisms underlying cellular processes such as gene expression, replication, and regulation. The course emphasizes hands-on laboratory training in cutting-edge molecular techniques such as PCR, gel electrophoresis, DNA Extraction and cloning, equipping participants with practical skills essential for research and diagnostics. With a focus on both theory and practical applications, this course prepares participants to apply molecular biology techniques in diverse scientific and medical fields.
Course Content:
- Introduction to Molecular Biology
- GMP & GLP
- Techniques of Sterilization & Decontamination
- Calculation of Molar Solutions, Buffer & Stock Solution Preparation
- Media Preparation
- Genomic DNA Extraction
- PCR
- Agarose Gel Electrophoresis
- Restriction Digestion
- Plasmid Isolation
- Competent Cell Preparation
- Transformation
- DNA conc. Determination & Purity Check
- Karyotyping
- DNA Extraction from Animal Cells
- RNA Isolation
- SDS-PAGE
Course Start Date: January 2, 2025
