Training on Recombinant DNA Technology

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THEORY
  • Introduction to RDT and Molecular Biology
  • Southern & Northern Blotting Techniques
  • Gene Cloning & Expression
  • Different Gene Delivery Methods
PRACTICAL
  • Media preparation
  • Isolation of Plasmid DNA (Classical method and kit based)
  • Isolation of Bacterial Genomic DNA (Classical method)
  • Isolation of Plant DNA – Cetyl trimethylammonium bromide (CTAB) mtd.
  • Bacterial Transformation (Classical method)
  • PCR
  • Karyotyping
  • Agarose Gel Electrophoresis
  • Qualitative and Quantitative estimation of DNA and RNA spectrophotometrically
  • Gel documentation and band intensity desitometry
  • Restriction digestion of DNA
  • Isolation of Genomic RNA
Amount - 11800